Hello everyone, I am currently working on a lab for biology and I need someone to explain how I would answer these questions. I am extremely confused right now and having trouble. Any help would be wonderful!
1. Translate the following sequence.
5’ AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3’
2. Create one or more point mutations in this sequence.
5’ AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3’
3. Translate your mutated sequence.
4. Now create a frameshift mutation by adding one or more bases.
5’ AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3’
5. Translate your mutated sequence.
6. Create a frameshift mutation by deleting one or more bases.
5’ AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3’
7. Translate your mutated sequence.
8. Align the amino acid sequences that you determined for each of the four transcripts.
1 ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____
2 ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____
3 ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____
4 ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____
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Answers & Comments
Verified answer
You need to read about protein translation. The questions will be easy once you understand translation from RNA to amino acids.
You might find this website useful.
https://www.khanacademy.org/science/biology/gene-e...
Otherwise I am sure you have a textbook. You can also search for other website or even youtube might have a teaching video.
If you just copy answers somebody might give you, you still won't understand it. And not knowing how much background you actually have, you can't expect people to explain it to you at the right level. If reading about the subject doesn't help, ask people in your class if they can help you. Or even ask the teacher whether you can get some extra help. It would be much better explained if there is actually communication from you to your teacher what exactly you have problems with.